Figure 1

Age-related dynamic changes in the percentage of ΔNp63⁺ and TAp63⁺ TECs, and ΔNp63 and TAp63 mRNA levels in WT murine thymi of various ages. (a) Representative IF staining of ΔNp63 (red) versus K8 (green) counterstaining, and (b) TAp63 (red) versus K8 (green) counterstaining in freshly isolated WT thymi of embryonic (E15 days) and postnatal mice at various ages. (c) Summary of ratios of % ΔNp63⁺ versus % K8⁺ TECs (dotted line) and % TAp63⁺ versus % K8⁺ TECs (solid line) in WT murine thymi of various ages, analyzed by Image-J software. Each data point includes 2 to 3 animals. (d) Summary of real-time RT-PCR results showing relative expression of ΔNp63 and TAp63 from FACS-sorted CD45⁻MHC-II⁺ TECs of young (∼2 months) and aged (≥18 months) WT mice. Each bar represents data (mean±S.E.M.) from five animals with three real-time RT-PCR repeats