Figure 2

Increase in senescent cell clusters with advancing age in the thymi of WT mice. Cryosections from freshly isolated thymic tissues of WT mice at various ages (a) were stained with SA-β-gal and counterstained with nuclear fast red. The senescent cell clusters are shown as blue spots. Images were taken at × 4 (left panels) and × 20 (right panels) magnifications of the objective lens; (b) were stained with TAp63 (green) and p21 (red, a cellular senescence marker). Right-most panels show TAp63 and p21 co-localization. The experiment was repeated three times with three animals at each age producing consistent results