Figure 6 | Cell Death & Disease

Figure 6

From: Expression of integrin alpha 10 is transcriptionally activated by pRb in mouse osteoblasts and is downregulated in multiple solid tumors

Figure 6

Effect of pRb on Integrin α10 protein levels and function. (a) MC3T3 pRb wild type and pRb-null cells were probed with antibodies against β-tubulin (2128S; Cell Signaling) and integrin α10 (AB6030; Millipore, Chicago, IL, USA) (green) and stained with DAPI (blue; nuclei). They were then examined using confocal immunofluorescence microscopy. Representative images are shown. (b) MC3T3 pRb wild type and pRb-null cells were incubated with either collagen IV or BSA substrate and allowed to adhere. Attached cells were permeabilized and stained with crystal violet. Absorbance at 540 nm was measured. Asterisk represents significant P-value: *P<0.05

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