Figure 1 | Cell Death & Disease

Figure 1

From: A feedback circuit between miR-133 and the ERK1/2 pathway involving an exquisite mechanism for regulating myoblast proliferation and differentiation

Figure 1

Overexpression of miR-133 repressed myoblast proliferation and promoted cell differentiation. (a and b) Expression of miR-133 in C2C12 cells after transfection with miR-133a mimics, miR-133b mimics or miR-133b inhibitor. MiR-133a mimics, miR-133b mimics, Duplex NC or miR-133b inhibitor were transfected into C2C12 cells, respectively. After transfection, cells were switched into the growth medium (GM) for 24 h. Then, total RNA of transfected cells was isolated, and the expression of miR-133 was analyzed by qPCR. (c) Overexpression of miR-133a/b repressed myoblast proliferation. C2C12 cells transfected with miR-133a mimics, miR-133b mimics or Duplex NC were collected for cell cycle analysis approximately 30 h after transfection. Cells were stained by propidium iodide and subjected to fluorescence-activated cell sorting analysis by flow cytometry. (d) Overexpression of miR-133a/b promoted C2C12 cell differentiation. Duplex NC, miR-133a mimics or miR-133b mimics were transfected into C2C12 cells, respectively. After transfection, cells were switched into the GM for 24 h. Then, cells were transferred to the differentiation medium (DM) for another 4 days before immunostaining for MyHC (green). Nuclei were stained with DAPI. (e) Area of myotubes stained in (d). Myotube area was calculated by Image Pro Plus software. Error bars represent S.D. from three independent experiments (*P<0.05; **P<0.01)

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