Figure 3 | Cell Death & Disease

Figure 3

From: A feedback circuit between miR-133 and the ERK1/2 pathway involving an exquisite mechanism for regulating myoblast proliferation and differentiation

Figure 3

Expression of miR-133, FGFR1, PP2AC and p-ERK1/2 during myoblast differentiation. (a) Morphologies of C2C12 cells cultured in the growth medium (0d), or differentiation medium for 1 day (1d), 2 days (2d) or 4 days (4d). (b) Expression of miR-133a and miR-133b during C2C12 differentiation. Total RNA of C2C12 cells collected at the four time points (0d, 1d, 2d and 4d) was isolated, and the expression of miR-133a and miR-133b was analyzed by Solexa sequencing. (c) Expression levels of FGFR1 and PP2AC mRNA during C2C12 differentiation did not significantly change, whereas the expression of miR-133 was significantly upregulated. Expression of miR-133 and other genes during C2C12 differentiation (using RNA samples from (b)) were analyzed by qPCR. (d) Protein of FGFR1 and PP2AC was downregulated during C2C12 differentiation, whereasile p-ERK1/2 was also downregulated. Total protein from C2C12 cells collected at the four time points (0d, 1d, 2d and 4d) was isolated, and protein expression of FGFR1, PP2AC and p-ERK1/2 was analyzed by western blotting, detecting tubulin and total ERK1/2 as controls. Error bars represent S.D. from three independent experiments (**P<0.01)

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