Figure 2 | Cell Death & Disease

Figure 2

From: Death of p53-defective cells triggered by forced mitotic entry in the presence of DNA damage is not uniquely dependent on Caspase-2 or the PIDDosome

Figure 2

Lack of Caspase-2 has no effect on spontaneous or IR-driven thymic lymphomagenesis in p53-null mice. (a) Kaplan–Meier analysis comparing tumor-free survival of p53+/− (median survival 492 days, n=16), p53−/− (median survival 158 days, n=28), p53+/−Casp2−/− (median survival 387 days, n=23) and DKO (median survival 129 days, n=8). (b) Kaplan–Meier analysis of tumor-free survival of wt (median survival 204 days, n=14), Casp2−/− (median survival 185 days, n=13), p53−/− (median survival 117 days, n=8) and DKO (median survival 116 days, n=16) mice after fractionated irradiation. Indicated P-values in (a and b) refer to differences compared with wt mice. (c) Freshly isolated thymic lymphoma cells from p53−/− and DKO mice were put in culture and treated with Gö6976 and/or exposed to IR. Cell survival was assessed after 24 h by AnnexinV/PI staining. (d) Thymic lymphoma cell lines derived from the indicated genotypes were treated with 0.1 μM or 1.0 μM Gö6976 alone, or in combination with increasing doses of IR. Bars represent means±S.E. of four independent experiments. *indicates significant differences (P<0.05) compared between p53−/− and DKO cells

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