Figure 4 | Cell Death & Disease

Figure 4

From: Death of p53-defective cells triggered by forced mitotic entry in the presence of DNA damage is not uniquely dependent on Caspase-2 or the PIDDosome

Figure 4

Cell death caused by Chk1 inhibition does not correlate with p53 status. Isogenic HCT116 cells lacking or expressing functional p53 and an shRNA targeting Caspase-2 were treated with Gö6796 and/or exposed to IR. Representative histograms from sub-G1 analysis performed after 48 h are shown in (a). Percentage of cell death was assessed by sub-G1-staining and flow cytometric analysis after 24 h and 48 h (b). Bars represent means±S.E. of ≥3 independent experiments per genotype and treatment. *Indicates significant differences (P<0.05) between controls and Dox-treated cells. (c) Caspase-2 expression was ablated by the addition of Doxycycline for 24 h prior exposure of cells to Gö or Gö+IR. Lysates for western blotting were harvested after additional 24 h. (*) In the image marks non-specific precipitates that leave the impression that IR triggers Caspase-3 activation. Higher magnification of the image will reveal the lack of the p17 fragment of active Caspase-3

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