Figure 1
FLIP and procaspase-8 expression in tumour samples; clinical correlates. (a) Automated tumour identification (‘region recognition module’) of lung adenocarcinoma tissue stained with FLIP antibody. (b) The ‘cellular detection module’: nucleus detection, nucleus quantification, cell boundary identification, segmentation of nucleus and cytosolic compartments and classification of cytosolic expression. (c) Absolute expression of FLIP and procaspase-8 in nuclei and cytoplasm across all patients. Significance determined by Mann–Whitney non-parametric, two-tailed t-test. (d) Correlations between tumoral expression of FLIP and procaspase-8 in nuclei (left) and cytoplasm (right), as determined using Pearson’s correlation. (e) Correlations of disease-free survival with the level of nuclear and cytoplasmic FLIP or procaspase-8 expression in tumour tissue across all patients. Statistical significance of Kaplan–Meier curves was determined by the log-rank (Mantel–Cox) test
