Figure 2

HDACi induce FLIP downregulation and apoptosis in NSCLC cell lines. (a) Western blot analysis of FLIP expression and PARP cleavage in H460 and A549 cells transfected with FLIP-targeted siRNA (FT) for 24 h. (b) Western blot analysis of FLIP expression and PARP cleavage in H460 and A549 cells treated with the indicated doses of vorinostat for 6 or 24 h. (c) Western blot analysis of FLIP and procaspase-8 expression and PARP cleavage in H460 and A549 cells treated with 5 μM vorinostat for the indicated timepoints. (d) Western blot analysis of basal FLIP, procaspase-8 and FADD expression in H460 and A549 NSCLC cells and 34LU normal lung fibroblasts. (e) Western blot analysis of FLIP and procaspase-8 expression and PARP cleavage in 34LU cells treated with vorinostat for 24 h. (f) Flow cytometric analysis of apoptosis (sub-G1 population) in H460, A549 and 34LU cells treated with vorinostat for the indicated times. Significant differences were determined by Student’s t-test: *P<0.05; **P<0.01; ***P<0.001