Figure 1 | Cell Death & Disease

Figure 1

From: miR-16 and miR-26a target checkpoint kinases Wee1 and Chk1 in response to p53 activation by genotoxic stress

Figure 1

Expression of miR-16 and miR-26a is induced in the presence of p53 by doxorubicin. (a) Comparison of the p53 levels in U2-OS cells stably expressing scrambled shRNA versus p53-specific shRNA (U2-OS KDp53) on treatment with doxorubicin. U2-OS cells with wild type and knocked-down expression of p53 were continuously treated with 0.5 μM of doxorubicin for 0, 6, and 12 h before harvesting. Cell lysates were then prepared and analysed by western blotting using p53-speicific antibody. Anti-tubulin serum was used as loading control. (b) Cell cycle distribution of U2-OS cells with wild type and knocked-down expression of p53 on doxorubicin treatment. Both cell types were treated with 0.5 μM of doxorubicin for 12 h, fixed with formaldehyde and stained with propidium iodine for subsequent cell cycle analysis. The same cells were treated with doxorubicin for 24 h and total RNA was extracted, converted to cDNA and analysed by real-time PCR for expression levels of miR-16 (c); miR-26a (d), and p21, as a positive control (e)

Back to article page