Figure 3 | Cell Death & Disease

Figure 3

From: miR-16 and miR-26a target checkpoint kinases Wee1 and Chk1 in response to p53 activation by genotoxic stress

Figure 3

Analysis of p53 binding to the regulatory regions of miR-16-2 and miR-26a genes by ChIP assay. (a) Shown is the scheme of the miR-26a-1 regulatory region. Putative p53-binding sites are depicted. Positions of nucleotides are given in respect to the transcription start site. (b) A summary of results of ChIP assay for p53 biniding on U2-OS scr (WT) and U2-OS shRNA-p53 (KD) cells. Cells were non-treated or treated for 24 h with doxorubicin and analysed for p53 binding to different amplicons of the sequence of the miR-26a-1 gene. (c) ChIP assay of Drosha and (d) p68 binding to the region −1500, where maximal p53 binding was detected. (e) Shown is the scheme of the SMC4 gene with intronic regulatory region for miR-16-2. (f) ChIP assay of p53 binding in the promoter of SMC4 gene and (G) in the upstream sequence of miR-16-2

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