Figure 5 | Cell Death & Disease

Figure 5

From: Wentilactone A as a novel potential antitumor agent induces apoptosis and G2/M arrest of human lung carcinoma cells, and is mediated by HRas-GTP accumulation to excessively activate the Ras/Raf/ERK/p53-p21 pathway

Figure 5

WA targets HRas to activate the Ras/Raf/ERK/p53-p21 pathway. (a) Total Ras, HRas-GTP, KRas-GTP, NRas-GTP and p-c-Raf (Ser338) protein levels were increased in both cells after 10 μM WA treatment. (b) Cells transfected with HRas siRNA or not were treated with or without 10 μM WA for 48 h, then cells were harvested for western blot assay against HRas-GTP, p-c-Raf, p-ERK. Apoptosis, cell-cycle analysis and related-proteins were also detected at 48 h. Values were means±S.D. of three independent experiments. *P<0.05, **P<0.01 versus control groups. (c) Binding affinity of WA to the HRas-GDP and HRas-GTP proteins determined by SPR. (d) 3D structure of interaction between WA and HRas-GDP (left), HRas-GTP (middle) or HRas-RasGAP (right) in detail. WA is represented by the stick model. Hydrogen bond was signed in red. (e) Starved cells were stimulated with 10% FBS or incubated with WA after 10 min FBS stimulation for the indicated time points. HRas-GTP levels were monitored using immunoblot assay

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