Figure 2
Exposure to 41 °C for 2 h inhibited replication and development of both early- and late-stage synchronised P. falciparum. Representative flow cytometry histograms (a) showed the progression of early (i) and late (ii) stage parasites kept at 37 °C, in comparison with early (iii) and late (iv) stage parasites exposed to 41 °C for 2 h. Time intervals are indicated on top, whereas synchronisation and treatment conditions are indicated on the right. (b) Stacked bar graphs show changes in parasitaemia (total bar height) and relative composition of the parasite population with respect to early- and late-stage parasites. A small portion of ring stage (Early) parasites exposed to 41 °C developed to late stages (Late), while most remained as ring-stage parasites. Late stages exposed to 41 °C failed to produce viable merozoites to infect erythrocytes and virtually no parasites were detected at 24 h, compared with 37 °C control parasites, which showed a large new ring population. Data points represent arithmetic mean±S.E.M. (n=4 for bi and n=5 for bii). Heat stress was applied at 0 h
