Figure 5
Heat stress-induced mitochondrial hyperpolarisation in late-stage parasites exposed to 40 °C for 24 h. Late-stage parasites exposed to 40 °C for 24 h showed mitochondrial hyperpolarisation after heat stress (i) and the following day (ii), whereas late-stage parasites exposed to 41 °C for 2 h showed practically no change in mitochondrial polarisation (iii and iv). (a) Overlays of DiOC6(3) fluorescence histograms show representative merged data sets of at least two replicates for each time point and condition as an individual peak. An increase in the median fluorescence of a population is indicated by a shift to the right (hyperpolarisation). Fluorescence values were taken as the median fluorescence of individual populations. (b) Bar graphs indicate the arithmetic mean of replicates±S.E.M. Comparisons between control and heat-stressed parasites showed P-values that are significant at <0.05 (*) or <0.001 (***). Heat stress conditions are indicated on the right
