Figure 5
Attenuation of PC migration under high glucose (HG) conditions. (a) In scratch wound assays, a significant portion of wound remained uncovered in PCs cultured under HG compared with normal glucose (NG) or osmolarity control (NG+L-Glu) conditions. The quantitative assessment of the data is shown below (n≥3; *P≤0.05 (NG versus HG or HG versus NG+L-Glu)). (b) A significantly lower number of PCs cultured under HG conditions migrated through the transwell membrane compared with PCs cultured under NG or NG+L-Glu conditions (n≥3; *P≤0.05 (NG versus HG or HG versus NG+L-Glu)). (c) Incubation of PCs cultured under HG with NAC restored basal migration. Incubation of retinal PCs under HG conditions with Rottlerin (PKC-δ inhibitor) or Y-27632 (ROCK I/II inhibitor) restored basal migration to levels seen in PCs cultured under NG or NG+L-Glu conditions (n≥3; *P≤0.05 (NG versus HG), **P≤0.05 (HG versus NG+L-Glu or HG+NAC or HG+Rottlerin or HG+Y-27632). (d) Incubation of PCs with PDGF-BB enhanced their migration under NG conditions. However, PDGF-BB had a minimal effect on migration of PCs cultured under HG. Although PCK-α inhibitor (Gö6976) restored basal migration of PCs cultured in HG, it did not restore migratory response to PDGF-BB. The inhibition of SHP-1 (SSG) in PCs cultured in HG restored both basal and PDGF-stimulated migration (n≥3; *P≤0.05 (NG versus HG), **P≤0.05 (HG versus HG+Gö6976 or HG+SSG))
