Figure 7
Incubation of PCs under high glucose (HG) conditions impacts their effect on EC function. (a) Conditioned medium was prepared from PCs incubated under normal glucose (NG), HG or control for 5 days. The effects of these conditioned medium on the migration of retinal EC was determined in a transwell assay. Please note a significant decrease in migration of retinal EC incubated with conditioned medium from PCs cultured in HG (n≥3; *P≤0.05 (NG versus HG and HG versus osmolarity control (NG+L-Glu)). (b) Retinal PCs were incubated under various glucose conditions for 5 days and used to assess their impact on retinal EC capillary morphogenesis in coculture (1 : 1) experiments. The mean number of branch points in 10 high power fields ( × 100) was determined. Please note a significant decrease in capillary morphogenesis of retinal ECs incubated with PCs under HG conditions (n≥3; *P≤0.05 (NG versus HG and HG versus NG+L-Glu)). Representative images for each condition are shown in (c)
