Figure 4

Dex abolished the anti-inflammatory effect of MSCs. (a) On day 63, serum levels of IFN-γ, TNF-α, IL-1β, and IL-12 in each group were assayed by Luminex technology. (b) On day 67, some mice were euthanized and liver sections from each group were stained with hematoxylin and eosin ( × 100 magnification). (c) Quantitative analysis of lymphoid infiltration in liver sections was carried out as described in Materials and Methods, and percentage of area affected by inflammatory infiltration was calculated. (d) Flow cytometric analysis of IFN-γ⁺, IL-17A⁺, and Foxp3⁺CD25⁺ T cells (CD4⁺) in the liver. (e) Quantitative analysis of IFN-γCD4⁺, IL-17A⁺CD4⁺ T cells, and Foxp3⁺CD25⁺CD4⁺ T cells in the liver. Values are shown as mean±S.E.M. and statistical significance indicated as *P<0.05, **P<0.01, and ***P<0.001. Representative of three independent experiments; n=7–10