Figure 6

iNOS^−/− MSCs did not suppress inflammation in mice suffering from liver fibrosis. (a) Liver histological sections from each group were stained with hematoxylin and eosin ( × 100 magnification). (b) Quantitative analysis of inflammatory cell infiltration in liver sections was carried out as described in Materials and Methods, and the percentage of area affected by inflammatory infiltration was calculated. (c) Flow cytometric analysis of IFN-γ⁺, IL-17A⁺, and Foxp3⁺CD25⁺ T cells (CD4⁺) in the liver. (d) Quantitative analysis of IFN-γ⁺, IL-17A⁺ cells, and Foxp3⁺CD25⁺ T cells (CD4⁺) in the liver. (e) Serum levels of IFN-γ. TNF-α, IL-1β, IL-12, IL-17A, and IL-10 in each group were assayed by Luminex technology. For this figure, values are shown as mean±S.E.M. and statistical significance indicated as **P<0.01, and ***P<0.001. Representative of three independent experiments; n=7–10