Table 1 The primer sequences using quantitative RT-PCR of M1 and M2 markers
From: Minocycline selectively inhibits M1 polarization of microglia
M1 microglia markers (5′→3′) | M2 microglia markers (5′→3′) | ||
|---|---|---|---|
CD86 | ACGATGGACCCCAGATGCACCA | Arginase 1 | TTAGGCCAAGGTGCTTGCTGCC |
GCGTCTCCACGGAAACAGCA | TACCATGGCCCTGAGGAGGTTC | ||
IL-1β | CCTGCAGCTGGAGAGTGTGGAT | CD206 | TCAGCTATTGGACGCGAGGCA |
TGTGCTCTGCTTGTGAGGTGCT | TCCGGGTTGCAAGTTGCCGT | ||
TNF-α | AGCCCACGTCGTAGCAAACCAC | Ym1 | ACCCCTGCCTGTGTACTCACCT |
AGGTACAACCCATCGGCTGGCA | CACTGAACGGGGCAGGTCCAAA | ||
CD68 | CCACAGGCAGCACAGTGGACA | IL-4 | TGGGTCTCAACCCCCAGCTAGT |
TCCACAGCAGAAGCTTTGGCCC | TGCATGGCGTCCCTTCTCCTGT | ||
IFN-γ | GCCAAGTTTGAGGTCAACAACCCA | IL-10 | GGCAGAGAACCATGGCCCAGAA |
CCCACCCCGAATCAGCAGCG | AATCGATGACAGCGCCTCAGCC | ||
