Figure 5 | Cell Death & Disease

Figure 5

From: miR-1207-5p and miR-1266 suppress gastric cancer growth and invasion by targeting telomerase reverse transcriptase

Figure 5

Biological effects of miR-1207-5p and miR-1266 on gastric cancer cells. SGC-7901 cells were infected with lentivirus packaged with different miRNAs. Each experiment was performed in triplicates. (a) Effects of miRNAs on cell proliferation. After the cells infected with the lentivirus were seeded in 96 wells for 18 h, they are transfected with pIRES2 or pIRES2-hTERT, CCK-8 assays were performed 48 h later. (b) Effects of miRNAs on cell cycle. Cells were treated the same as (a) and flow cytometry were performed. (c and d) Effects of miRNAs on the invasion of gastric cancer cells. Cells were treated the same as in (a) and then they were seeded into the Matrigel invasion chamber. Subsequently, the chambers were transferred to wells filled with culture medium containing 10% FBS. After 48 h of incubation, the number of invasive cells from five random areas of the membrane was counted using light microscopy. Both miR-1207-5p and miR-1266 significantly suppressed cell invasion. The mean of triplicate experiments±S.D. is shown (*P<0.05, **P<0.01, one-way ANOVA)

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