Figure 7
Both miR-1207-5p and miR-1266 inhibit the growth of transplanted SGC-7901 cells in nude mice. SGC-7901 cells (3 × 106) were injected s.c. into the left and right axillae of nude mice. When the tumors reached ∼100 mm3 in size (8 days after the injection of tumor cells), 0.1 ml Lv-miR-1266/1207-5p or Lv-cel vector suspension (107 pfu/ml) was injected intra-tumorally into the center of the tumor masses. Six weeks later, the tumors were visualized and harvested. Three mice were used for each group. (a) The tumors in vivo were photographed with an In-Vivo Imaging System. Left: Lv-cel vs. miR-1207-5p groups; right: Lv-cel vs. Lv-miR-1266 groups. (b) The harvested transplanted tumors. (c) The weight of the harvested transplanted tumors. (d) The expression of miRNAs and hTERT in the tumors revealed by real-time RT-PCR (left panel) or western blot (right panel) respectively. The optical density ratio refers to mean value of the target gene/GAPDH ratio from three independent experiments. (e and f) Immunohistochemistry for cell proliferation markers of PCNA (e) and Ki-67 (f), respectively
