Figure 1
From: MiR-216a: a link between endothelial dysfunction and autophagy
Autophagy is impaired in HUVECs during aging. (a) RT-PCR of endogenous miR-216a (n=10 per group), BECN1 and ATG5 mRNA levels (n=6) and (b) BECN1, ATG5 and LC3B protein levels (n=4) in HUVECs aging model. (**P<0.005, ***P<0.0005. Student’s t-test, data are mean ± S.E.M.). (c) miR-216a dose-dependent suppression of luciferase activity in HEK293 cells transfected with pGL3-BECN1-3′UTR vector containing miR-216a consensus binding sequences in the BECN1-3′-UTR, or pGL3-BECN1mut-3′UTR vector containing mutated miR-216a consensus binding sequences in the BECN1-3′-UTR (n=4). (**P<0.005, ***P<0.0005 miR-216a transfected cells compared with controls) by one-way ANOVA. Data are mean ± S.E.M.
