Figure 5
From: MiR-216a: a link between endothelial dysfunction and autophagy
miR-216a overexpression blocked ox-LDL-induced autophagy and regulates ox-LDL accumulation and monocytes adhesion in PDLs8 HUVECs. (a) Cyto-ID autophagy detection expressed as a percentage of total viable cells (n=6). (b) LC3B immunofluorescence showing LC3B-II positive signals with punctuate structures in control cells under ox-LDL treatment and a reduction in ox-LDL miR216a transfected cells. Scale bar, 60X. Fluorescence intensity from experiment derived from average of 35 cells in four images per group. (**P<0.005, one-way ANOVA. Data are mean ± S.E.M.). PDLs8 HUVECs transfected with miR-216a and treated with Dil-ox-LDL or ox-LDL were analyzed for: (c) Dil-ox-LDL fluorescence (***P=0.0002 Student’s t-test, data are mean ± S.E.M.) and d, THP-1 monocyte adhesion. (*P<0.05, **P<0.005, one-way ANOVA. Data are mean ± S.E.M.)
