Figure 1

Gene expression of VE-Cad⁺ derived cells after postnatal Cre activation. (a) IF performed on TA of Cdh5-CreER^T2; R26R-EYFP mice activated with TAM injection at postnatal days (P) 6–8, at P11 and P60. Representative images of muscle cross-sections immunostained with antibodies specific for CD31 (in red), EYFP (in green), showing colocalization of labeled cells with CD31. Magnification × 20. Scale bar: 50 μm. (b) Gating strategy for FACS sorting of EYFP⁺ cells from muscle; 2D density plot showing FS versus SS, delimits a tight region around the live cell population (R1). A second density plot, gated only on events encircled within R1, shows EYFP⁺ cells on the x-axis and SS on the y axis (R2). EYFP⁺ cells are selected and sorted. Cells are used for RNA extraction, at P11 and P60. (c) Real-time PCR performed on cDNA obtained from RNA of EYFP⁺ cells sorted from P11 and P60 Cdh5-CreER^T2; R26R-EYFP mice activated with TAM injection at postnatal days (P) 6–8. The data are normalized on cyclophillin A expression