Figure 3 | Cell Death & Disease

Figure 3

From: RETRACTED ARTICLE: OCT4 as a target of miR-34a stimulates p63 but inhibits p53 to promote human cell transformation

Figure 3

OCT4 is suppressed by miR-34a in human embryo stem cells (hESCs). (a) The miR-34a-3p level in hESCs, H9. H9 cells were transfected with either control RNA or miR-34a mimics as described in Materials and Methods. The miR-34a-3p levels were measured at different times after transfection using a real-time PCR approach in triple sets, and the data were normalized with internal control RNA, RNU48. (b) Immunoblots of whole-cell lysates from H9 cells measured at different times after transfection with control RNA or miR-34a mimics. Actin was used as an internal loading control. (c) Photomicrographs of H9 cells at 48 h after transfection with either control RNA or miR-34a mimcs. DAPI, 4,6-diamidino-2-phenylindole

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