Figure 6

Fragmentation of the GA in the motor neurons of tgMT mouse spinal cords. Cross-sections of 7-month-old tgWT, tgMT and non-transgenic control (Ctrl) mouse spinal cord was colabeled with anti-Myc antibody for transgenic Seipin (green) and anti-Golgi (a), GM130 antibody against GA (b) or COPII antibody against transport vesicles from rough ER to GA (c). In contrast to the well-defined structures in the motor neurons of tgWT or control mice, the motor neurons in tgMT mice showed discrete GA fragments, and aggregated or dispersed distribution of COPII vesicles (circles). Scale bars=10 μm