Figure 4 | Cell Death & Disease

Figure 4

From: Anti-inflammatory effects of a Chinese herbal medicine in atherosclerosis via estrogen receptor β mediating nitric oxide production and NF-κB suppression in endothelial cells

Figure 4

BSNXD suppresses adhesion molecules expression on HUVECs through ERβ/NO/NF-κB pathway. The primary HUVECs were exposed to control serum or 10% BSNXD-derived serum for 48 h; in the final 24 h culture the ox-LDL was added. The supernatants were collected, and MCP-1 concentrations were determined. The MCP-1 expression of HUVECs was determined by western blot analysis. The mRNA and protein expression levels of cell adhesion molecules (ICAM-1, VCAM-1 and E-selectin) were assessed by RT-PCR, western blot and FACS. The MCP-1(a), ICAM-1 (b), VCAM-1 (c) and E-selectin (d) expression was greatly decreased after treatment with the 10% drug-derived serum. NOS inhibitor (L-NAME), ERβ antagonist (R, RTHC) other than ERα antagonist (MPP) could block these effects induced by the drug-derived serum. Data are expressed as mean values±S.E.M. (n=6). *P<0.05, **P<0.01

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