Figure 5
From: Zebularine regulates early stages of mESC differentiation: effect on cardiac commitment
Zebularine treatment caused (a) an increment of global demethylase activity and a decrease of DNTMs activity (b) a decrease of HMT (H3K9) and increase of H3K4 (c) reduction of global HDAC activity. Blots are representative of 4 independent experiments. (d) Western blot detection after zebularine treatment of acetylated state of histone H4, and methylated state of histones H3K4me3 and H3K9me1, β-actin was used as loading control
