Figure 6
From: HDAC inhibitors induce tumor-cell-selective pro-apoptotic transcriptional responses
Regulation of BCL2 family gene expression by the HDACi romidepsin and the DNA damaging agent etoposide. BJ (left panels) and BJ LTSTERas (right panels) fibroblasts were treated for 0, 4, 12 and 24 h with 50 nM romidepsin (red), 50 μM etoposide (blue) and analyzed for the expression of BAK1, BCL2L11, BIK, BMF and BCL2A1 by qRT-PCR. Expression of each gene was calculated relative to the non-romidepsin- and non-etoposide-responding control gene RPL32 and fold-change expression over time was calculated relative to time 0 h. Gene expression changes resulting from vehicle treatment (black) are also shown. Data are presented as the mean expression fold change from two independent time course experiments
