Figure 2

Representative microphotographs of the DG of the hippocampus stained for BrdU. A single IR dose of 8 Gy or sham-IR was administered to the brains of P14 mice and BrdU-labeled NSPCs were injected into the hippocampus 1 day (P15) (a and b), 1 week (P21) (c and d) or 6 weeks (P56) (e and f) after IR. At 5 weeks after grafting, we evaluated the surviving BrdU-positive cells in the GCL of the DG in the hippocampus. It was apparent that the majority of the grafted, surviving cells were found in the dorsal blade of the GCL, and that survival was lower in the irradiated GCL 1 day after IR. Scale bar=100 μm