Figure 7

Astrogliosis and reduced thickness of the dorsal GCL blade. Representative microphotographs showing BrdU-labeled NSPCs grafted on P15 and evaluated 5 weeks after grafting. Cells were stained for BrdU (a, green), S100β (b, blue) and NeuN (c, red). An overlay of the three stainings is shown in (d). As seen in Figure 3, the majority of the grafted BrdU+ cells were found in the dorsal blade. Phenotyping revealed that approximately half of the BrdU+ cells developed into S100β+ astrocytes, depending on the time of grafting (Figure 6), and these BrdU+/S100β+ were almost exclusively found in the dorsal blade. The reduced thickness of the dorsal blade is most easily appreciated in the NeuN staining (c). Scale bar=25 μm