Figure 1 | Cell Death & Disease

Figure 1

From: AF-MSCs fate can be regulated by culture conditions

Figure 1

Dedifferentiation potential of AL cells. (a) (i) SSEA-4 expression levels were examined by FACS analysis, and Sox-2, Oct-4 and Nanog expression levels by Real-time PCR analysis genes during adipogenic induction of AF-MSCs. (ii) AF-MSCs and DAF-MSCs induced at different time points were analyzed for the expression of SSEA-4 by FACS analysis and for the expression of Sox-2, Oct-4 and Nanog by real-time PCR analysis. Data are presented as the mean±S.D. of three independent experiments, and were statistically analyzed by one-way ANOVA. (b) (i) Morphology of AF-MSCs, AL cells and DAF-MSCs in culture, and (b) (ii) after Oil Red O staining. (c) Semiquantitative PCR analysis for PPARγ and LPL on AF-MSCs, AL cells and DAF-MSCs. Data are presented as the mean±S.D. of three independent experiments and were statistically analyzed by one-way ANOVA

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