Figure 3

2DE analysis of AF-MSCs, AL cells and DAF-MSCs. (a) Representative 2DE gel image of proteins identified from (i) AF-MSCs, (ii) AL cells and (iii) DAF-MSCs. The protein spots in each population are indicated with their abbreviated names listed in Supplementary Table 2. (b) Comparative expression analysis for: (i) LEG-1, (ii) PHB, (iii) EIF3I 3 subunit, (iv) VIME, (v) ATPB subunit-β, (vi) RCN3, (vii) PDIA1 and (viii) GSTP1. (c) Western blot analysis in cell extracts from AF-MSCs, AL cells and DAF-MSCs for (i) GSTP1 P1 and (ii) PHB expression. Protein bands of 32 and 26 kDa corresponding to the two above proteins were detected. β-actin was used as positive control for equal loading