Figure 5 | Cell Death & Disease

Figure 5

From: AF-MSCs fate can be regulated by culture conditions

Figure 5

Stem cell marker expression and metabolic activity of AF-MSCs, AL cells, TRAF-MSCs and HL cells. (a) Real-time PCR expression analysis for (i) Oct4, (ii) Sox-2 and (iii) Nanog in AF-MSCs, AL, TRAF-MSCs and HL cells. Data are presented as the mean±S.D. of three independent experiments, and the statistical significance was defined by one-way ANOVA. (b) (i) Microscope photos of labeled lysosomes (red) and mitochondria (green) on AF-MSCs, AL cells, TRAF-MSCs and HL cells. (ii) Ratios of MitoTracker/LysoTracker for the four cell types. Data are presented as the mean±S.D. of 10 images per cell category, and were statistically analyzed by one-way ANOVA. (c) Semiquantitative PCR analysis for the expression of (i) the adipogenic markers, FABP4, PPARγ and LPL and (ii) the hepatogenic marker, tryrosine aminotransferase in AF-MSCs, AL, TRAF-MSCs and HL cells. Tyrosine aminotransferase was expressed in similar levels in AF-MSCs and TRAF-MSCs (0.05<P<0.1, Student’s t-test). Data are presented as the mean±S.D. of three independent experiments and the statistical significance was defined by one-way ANOVA

Back to article page