Figure 5
From: ATP/P2X7 axis modulates myeloid-derived suppressor cell functions in neuroblastoma microenvironment
P2X7R-associated plasma membrane permeability changes in M-MDSCs and G-MDSCs from NB-bearing mice. Panels a and c show BzATP-mediated induction of plasma membrane depolarization in Ca2+-containing medium. Changes in plasma membrane potential were measured with bisoxonol as described in Materials and Methods. One representative experiment out of three performed with similar results is shown. Panel a shows the tracers of TF G-MDSCs (black trace), NB G-MDSCs (orange trace), TF G-MDSCs (red trace) and NB G-MDSCs (purple trace) pre-treated with oATP. Panel b shows the tracer of TF M-MDSCs (green trace), NB M-MDSCs (blue trace), TF M-MDSCs (pink trace) and NB M-MDSCs (cyan trace) pre-treated with oATP. Panel c shows the tracers of HEK293-P2X7R (brown trace) and HEK293-Mock (gray trace) cells used as positive and negative control respectively. Panels d and e show Bz-ATP-mediated induction of EtBr uptake. Panel d shows the tracers of TF G-MDSCs (black trace) and NB G-MDSCs (orange trace); panel e shows the tracers of TF M-MDSCs (green trace) and NB M-MDSCs (blue trace). One representative experiment out of three performed with similar results is shown
