Figure 7

P2X7R-dependent IL-1β secretion, ARG-1 expression and LDH release from MSC-1 and MSC-2 lines. For IL-1β release (panels a and b), cell lines were primed for 4 h with 1 μg/ml LPS and then stimulated for 30 min with BzATP. Cell-free supernatants were assayed for IL-1β concentration. For ARG-1 expression (panels c and d), TGF-β1 secretion (panels e and f) and ROS production (panels g and h), the cells were primed with 1 μg/ml LPS for 4 h, followed by stimulation with 100 or 300 μM BzATP for 30 min and 4 h BzATP stimulations were performed also without the priming with LPS. For LDH release (panels i and j), cells were incubated at 37 °C for 8 h in the presence of increasing ATP concentrations. Enzyme activity is expressed as % of total activity released by Triton X-100