Figure 3 | Cell Death & Disease

Figure 3

From: Temozolomide resistance in glioblastoma cells occurs partly through epidermal growth factor receptor-mediated induction of connexin 43

Figure 3

Effect of TMZ on the 5′ regulatory region of GJA1 (Cx43). T98G and U87 cells were transfected with luciferase reporter vectors with various inserts, upstream of the transcriptional initiation site of the GJA1 5′ regulatory region.27 The transfectants were treated with vehicle or 200 μM TMZ for 72 h. Whole-cell lysates from the viable cells were analyzed for luciferase activity. The relative expression in luciferase activities are presented as the mean±S.D. The data represent triplicates from four independent experiments, n=12 (a). Shown is the AP-1 site within the 5′ regulatory region Cx43 (GJA1) (b). GBM cell lines were transfected with a reporter vector containing AP-1 Tandem Repeats. The transfectants were treated with 200 μM TMZ. After 72 h, whole-cell extracts were studied for luciferase levels. The results are presented as mean±S.D. of triplicates in four independent experiments, n=12 (c). *P<0.05 versus vehicle

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