Figure 4 | Cell Death & Disease

Figure 4

From: Depletion of pre-mRNA splicing factor Cdc5L inhibits mitotic progression and triggers mitotic catastrophe

Figure 4

Downregulation of three other components of the hPrp19/Cdc5L complex leads to chromosome misalignment in mitosis. (a–f) HeLa/GFP-H2B cells were transfected with control siRNA or siRNAs against Prp19 (a and b), SPF27 (c and d), PLRG1 (e and f), respectively. Representative time-lapse images are shown (a, c, e). The time on the images is in minutes. A, anaphase; M, metaphase; NEB, nuclear envelope breakdown. Scale bar, 10 μm. The percentage of mitotic cells with misaligned chromosome was analyzed in control and Prp19-knockdown cells (b), SPF27-knockdown cells (d) or PLRG1-knockdown cells entering mitosis (f). Data are shown as mean±S.D. (g) Immunoblot analysis of HeLa cells transfected with control siRNA or siRNA against Prp19, SPF27, PLRG1, respectively

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