Figure 5 | Cell Death & Disease

Figure 5

From: Depletion of pre-mRNA splicing factor Cdc5L inhibits mitotic progression and triggers mitotic catastrophe

Figure 5

Cdc5L regulates the expression and splicing efficiency of a set of genes involved in mitotic progression and the DNA damage response. (a) HeLa cells transfected with control and Cdc5L siRNA were analyzed by gene expression profiling. All genes altered by Cdc5L knockdown are shown (1.5-fold, P<0.05). (b) Analysis of molecular and cellular functions that showed significant changes after Cdc5L knockdown by Ingenuity Pathway Analysis software. (c) Validation of altered gene expression induced by Cdc5L knockdown in a subset of genes including DYNC1H1, DYNLRB2, DCTN4, MYB, RAD1, BARD1,Cdc5L, DCTN1 by qRT-PCR. Gene expression was normalized to GAPDH levels. Data are shown as mean±S.D. (d) Schematic diagram of the primer pairs used to detect spliced and unspliced mRNAs (top panel). Quantitative real-time PCR analysis was used to determine the ratio of spliced to unspliced of mRNA for DYNC1H1, DCTN4, RAD1, DYNLRB2 in control or Cdc5L-knockdown cells (bottom panel)

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