Figure 1

Characterization of iPS cells bioengineered to express MMP9 or PlGF. (a, left) RT-PCR showing the presence of an MMP9 transcript in iPS cells bioengineered to secrete MMP9 (MiPS) into the culture medium; (a, right) RT-PCR illustrating that the PlGF transcript was produced by iPS cells engineered to secrete PlGF (PiPS). iPS cells transduced with an nLacZ viral vector (iPS) were used as a negative control. (b) Cell invasion experiments attested an enhanced aptitude of MiPS cells, with respect to control iPS cells, to permeabilize the matrix and migrate when attracted by either SCF or SDF-1. The graph gives the number of invading cells/field on the y axis. BSA (bovine serum albumin) was used as the control condition. Mean±S.E.M.; N=3. (c) Representative images of DAPI-stained nuclei in invasion experiments. iPS and MiPS cells were stimulated to migrate through a matrigel membrane by either SCF or SDF-1. Magnification × 20; white bars=20 μm. (d, left) Number of capillary-like structures generated by HUVECs after exposure to conditioned media; (d, right) length of the newly formed capillaries after exposure to conditioned media. Mean±S.E.M.; N=3. (e) Representative images of capillary-like structures formed by HUVECs after exposure to control iPS- or PiPS cell-conditioned media. Magnification × 5; white bars=100 μm