Figure 2 | Cell Death & Disease

Figure 2

From: PlGF–MMP9-engineered iPS cells supported on a PEG–fibrinogen hydrogel scaffold possess an enhanced capacity to repair damaged myocardium

Figure 2

Effect of growing iPS cells on PEG–fibrinogen scaffolds. (a) Morphology of iPS, MiPS, and PiPS cell colonies cultured on mouse embryonic fibroblast (MEF) feeder layers (upper row), on PEG–fibrinogen (PF) scaffolds without a feeder layer (second row), or on PF supplemented with additional (1 and 2%) PEG–diacrylate (PEG–DA) in the absence of MEFs (lower two rows). White bars=100 μm. (b) qRT-PCR attesting the expression of three embryonic genes after 7 days and 14 days of culture. iPS, MiPS, and PiPS cells maintain their pluripotent status when grown on MEF (CNTR MEF), PF, or 1 and 2% PEG–DA-enriched PF. Bar graphs express ΔΔCt values. Mean±S.E.M.; N=4. (c) Immunofluorescence for SSEA1 in iPS, MiPS, and PiPS cells after 14 days of culture on PF supplemented with an additional 1% PEG–DA. Nuclei were stained with DAPI. White bars=100 μm

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