Figure 5 | Cell Death & Disease

Figure 5

From: Arginine vasopressin neuronal loss results from autophagy-associated cell death in a mouse model for familial neurohypophysial diabetes insipidus

Figure 5

Autophagosome formation and p62 expression under ER stress in mouse hypothalamic slice explants. (a) Immunofluorescence staining for GFP in hypothalamic slice explants of GFP-LC3 transgenic mice cultured with vehicle (control) and 1 μM thapsigargin for 24 h. (b) The number of GFP-LC3 puncta per cell. GFP-LC3 puncta in 60–70 cells were counted in each group in a series of equally acquired images. (c) Quantitative real-time RT-PCR analysis for p62 mRNA in hypothalamic slice explants of WT mice cultured with vehicle (veh) and 1 μM thapsigargin for 6, 12 and 24 h. (d) Immunoblot analysis of protein lysates from hypothalamic slice explants of WT mice immunolabeled for p62. Explants were incubated with vehicle (veh) and 1 μM thapsigargin for 6, 12 and 24 h. Results are expressed as means±S.E. *P<0.05, ***P<0.001 compared with vehicle-treated explants. Scale bars, 10 μm

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