Figure 2

14-3-3γ is selectively upregulated in cerebral cortical neurons in 1 h-pMCAo rats. (a) Representative ischemic rat brain slice after TTC staining showing the ipsilateral penumbra area (Ipsi, indicated by the black box) and its Contra for 14-3-3 isoform measurements. (b) Representative micrographs of double-fluorescent immunostaining showing the expression of 14-3-3γ in cerebral cortical neurons in the Ipsi of 1 h-pMCAo. NeuN is a marker of neurons. Arrows indicate neurons co-expressing 14-3-3γ and NeuN. (c) Representative micrographs of IHC showing the elevation of 14-3-3γ in the Ipsi of 1 h-pMCAo rat. (d) Statistical analysis of relative expression levels of 14-3-3 isoforms in the Ipsi of 1 h-pMCAo rats. The average intensity of 14-3-3 isoform was measured from more than 100 neurons in each IHC micrograph by using Plus software. The relative expression level of 14-3-3 isoform in the Ipsi was compared with that of Contra. *P<0.05 versus its Contra (n=7). (e) Representative results of RT-PCR (left panel) and statistical analysis of qPCR (right panel) of 14-3-3 isoforms in the Contra and Ipsi of 1 h-pMCAo rats. Relative 14-3-3 level was normalized to that of β-actin. *P<0.05, **P<0.01 versus Contra (n=6/group). (f) Representative western blot results (left panel) and statistical analysis (right panel) of 14-3-3 isoforms in the Contra and Ipsi of 1 h-pMCAo rats. Relative 14-3-3 level was normalized to that of β-actin. *P<0.05, **P<0.01 versus Contra (n=6/group)