Figure 5 | Cell Death & Disease

Figure 5

From: Salt-inducible kinase 3 is a novel mitotic regulator and a target for enhancing antimitotic therapeutic-mediated cell death

Figure 5

Downregulation of SIK3 increases the cytotoxicity of spindle poisons. (a) SIK3 depletion increases nocodazole-mediated mitotic cell death in HeLa cells. HeLa cells expressing histone H2B-GFP were transfected with control small interfering RNA (siRNA) or siSIK3. After 24 h, the cells were treated with 6.25 ng/ml of nocodazole. After 2 h, individual cells were tracked using time-lapse microscopy for 24 h. Each horizontal bar represents one cell (n=50). Key: gray=interphase; black=mitosis (from DNA condensation to anaphase or cell death); truncated bars=cell death. (b) The duration of mitosis after nocodazole treatment is increased after SIK3 depletion. HeLa/H2B-GFP cells were transfected, challenged with nocodazole, and imaged with time-lapse microscopy as described in a. The duration from prometaphase to metaphase and from metaphase to anaphase was quantified (average±90% CI). Transfection of siSIK3 significantly increased the duration of mitosis after nocodazole challenge (P<0.0001; unpaired t-test)

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