Figure 3
From: Identification of ML-9 as a lysosomotropic agent targeting autophagy and cell death
ML-9 affects maturation of autophagosomes and increases lysosomal pH. (a) LNCaP cells were transiently transfected with mCherry-GFP-LC3B and treated with full, serum-starved, or ML-9 (30 μM) containing medium for 12 h. The colocalization of GFP and mCherry signals was analyzed. Quantitation shown on the right represents means±S.D. mCherry-positive/GFP-negative puncta per cell (n=20). (b) ML-9 decreases lysosomal acidification. LNCaP cells were treated with full, serum-starved, 30 μM ML-9-containing full medium or 30 μM ML-9-containing serum-starved medium for12 h, stained with acridine orange and analyzed using confocal microscopy
