Figure 4

Adipogenic differentiation of PDGFRα⁺ cells and myogenic differentiation of CD56⁺ cells after adipogenic induction. (a) The three populations indicated were sorted from human muscle-derived cells and cultured in growth condition for 3 days, and then cells were subjected to adipogenic condition. Cells were stained with antibodies against C/EBPα and peroxisome proliferator-activated receptor-γ (PPARγ) or Oil Red O. The percentages of positive cells are shown in the panels as means±s.d., n=15 fields from three independent preparations. Scale bar: 50 μm. (b) Adipogenic differentiation was evaluated by quantifying the intensity of Oil Red O staining. Values are represented as the ratio to CD56⁺ cells and shown as means±s.d. of eight independent preparations. *P<0.01, **P<0.05. (c) Reverse transcription-polymerase chain reaction (RT-PCR) analysis of indicated genes in undifferentiated PDGFRα⁺ cells and PDGFRα⁺ cell-derived adipocytes. (d) After adipogenic induction, the three populations indicated were stained with antibodies against MyoD and MyHC. Scale bar: 50 μm