Figure 5
From: Osteopontin deficiency aggravates hepatic injury induced by ischemia–reperfusion in mice
OPN silencing sensitized AML12 hepatocytes to cell death induced by TNFα. After silencing of OPN with siRNA in AML12 cells, the cell viability (MTT assay) (a) and LDH release (b) were evaluated in response to TNFα (20 ng/ml) for 16 h in DMEM, 4.5 g/l glucose supplemented with 0.5% BSA (n=3). As OPN silencing affected the basal cell viability, the results were expressed as a function of the corresponding basal state (si Ctr or si OPN, respectively) and as means±S.E.M. Data were statistically analyzed using Student’s t-test. *P<0.05
