Figure 5
From: Receptor-interacting protein kinases modulate noise-induced sensory hair cell death
Noise-induced elevation in CC8 is inhibited by ZVAD, but not by Nec-1 or siRIP3 treatment, while noise-induced activation of CC9 in sensory hair cells increases by pretreatment with siRIP3. (a) CC8-associated immunofluorescence in the cochlear epithelium. Control: baseline levels of CC8 without noise exposure. Noise: immunofluorescence associated with CC8 increases 1 h after 106 dB SPL noise exposure. Noise+ZVAD: treatment with ZVAD via local delivery inhibits noise-induced elevation of CC8. Noise+Nec-1: treatment with local delivery of Nec-1 does not influence levels of noise-induced CC8. Green: phalloidin labeling of sensory hair cells; red: CC8. Images are taken from the upper basal turn and are representative of five ears for each condition. Scale bar=10 μm. (a′) Quantification of CC8-associated fluorescence in OHCs confirms a significant increase after noise exposure. Noise-induced CC8 is blocked by local delivery of ZVAD but is not influenced by Nec-1 treatment. Data are presented as means+S.D.; n=5; *P<0.05, **P<0.001. (b) There is no difference in CC8-associated immunofluorescence between 106 dB SPL noise-exposed siRIP3 and siControl samples. (c) Induction of CC9 by noise exposure is increased by treatment with siRIP3. Green: phalloidin labeling of sensory hair cells; red: CC8 or CC9. Images are taken from the upper basal turn and are representative of five ears for each condition. Scale bar=10 μm. (c′) Quantification of CC9-associated immunofluorescence in OHCs confirms a significant increase after siRIP3 treatment. Data are presented as means+S.D.; n=5; *P<0.05, **P<0.001
