Figure 6
From: Receptor-interacting protein kinases modulate noise-induced sensory hair cell death
siRIP3 treatment increases noise-induced EndoG translocation into nuclei of OHCs. (a) Immunolabeling of EndoG in OHCs of cochlear epithelia. siControl: EndoG is localized to the cytosol of OHCs. siControl+Noise: EndoG is found in a few nuclei 1 h after 106 dB SPL noise exposure (arrows). siRIP3+Noise: silencing RIP3 increases noise-induced EndoG translocation into nuclei (arrows). Red: PI staining for nuclei; green: EndoG. Images are taken from the cochlear lower basal turn and are representative of five ears for each condition. (b) Quantification of OHCs with EndoG-positive nuclei in the lower basal turn confirms a significant increase with siRIP3 treatment. Data are presented as means+S.D.; n=5; *P<0.05
