Figure 1
cDKO mice were born but died prematurely. (a) PCR analysis of genomic DNA from the tails of Meox2+/+PLCδ1fl/−PLCδ3+/− (Meox2+/+δ1fl/−δ3+/−), Meox2cre/+PLCδ1fl/−PLCδ3+/− (Meox2cre/+δ1fl/−δ3+/−), Meox2+/+PLCδ1fl/−PLCδ3−/− (Meox2+/+δ1fl/−δ3−/−), and Meox2cre/+PLCδ1fl/−PLCδ3−/− (Meox2cre/+δ1fl/−δ3−/−) mice. W, M, and F indicate PCR products from wild-type, mutant, and floxed alleles, respectively. (b) Hematoxylin and eosin staining of the labyrinth area of the placenta from Meox2+/+PLCδ1fl/−PLCδ3+/− (Meox2+/+δ1fl/−δ3+/−), Meox2cre/+PLCδ1fl/−PLCδ3+/− (Meox2cre/+δ1fl/−δ3+/−), Meox2+/+PLCδ1fl/−PLCδ3−/− (Meox2+/+δ1fl/−δ3−/−), and Meox2cre/+PLCδ1fl/−PLCδ3−/− (Meox2cre/+δ1fl/−δ3−/−) embryos at E13.5. The arrows indicate embryonal vessels and the arrowheads indicate maternal vessels. Scale bar, 25 μm. (c) Immunoblotting of PLCδ1, PLCδ3, and tubulin in tissues from Meox2+/+PLCδ1fl/−PLCδ3+/− (Meox2+/+δ1fl/−δ3+/−) and Meox2cre/+PLCδ1fl/−PLCδ3−/− (Meox2cre/+δ1fl/−δ3−/−) mice. (d) Survival curves for Meox2+/+PLCδ1fl/−PLCδ3+/− (Meox2+/+δ1fl/−δ3+/−), Meox2cre/+PLCδ1fl/−PLCδ3+/− (Meox2cre/+δ1fl/−δ3+/−), Meox2+/+PLCδ1fl/−PLCδ3−/− (Meox2+/+δ1fl/−δ3−/−), and Meox2cre/+PLCδ1fl/−PLCδ3−/− (Meox2cre/+δ1fl/−δ3−/−) mice (Meox2+/+PLCδ1fl/−PLCδ3+/−, n=70; Meox2+/+PLCδ1fl/−PLCδ3−/−, n=64; Meox2cre/+PLCδ1fl/−PLCδ3+/−, n=62; and Meox2cre/+PLCδ1fl/−PLCδ3−/−cDKO, n=62)
